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potential new approaches to stimulate glp-2 secretion and intestinal adaptation in weanling piglets

category: feed additives

date:june 2012

authors: ipharraguerre i.r., burrin, d.g., mereu, a., menoyo, d., holst, j.j. and tedo, g.

book/journal:12th international symposium - digestive physiology of pigs. keystone, co, usa, may 29 –june 1

abstract:

weaning is a stressful event characterized by a transient period of anorexia and intestinal atrophy. the weaning-induced intestinal atrophy may be partly mediated by reduced secretion of glucagon-like peptide 2 (glp-2), a trophic gut peptide secreted in response to luminal nutrition. we conducted 2 trials to investigate the hypothesis that stimulating endogenous glp-2 secretion immediately after weaning may improve intestinal integrity in piglets. treatment solutions were chenodeoxycholic acid [60 mg/kg bw, (cdc)] and β-sitoesterol [100 mg/kg bw, (bse)] in trial 1, and cdc (120 mg/kg bw) and zein protein hydrolyzate (1.4 g/kg bw) in trial 2. in each trial, 36 piglets were weaned on average at 22 d of age and 6.1 kg of bw, distributed among treatments (12 pigs/treatment) in individual pens, and fed ad libitum a prestarter diet. during the first 6 d after weaning, feed intake was recorded daily and all piglets were intragastrically infused once daily at 7 pm with 50 ml of either water (control) or treatment solutions. on d 5 plasma was obtained from 6 pigs/treatment at -15, 0, 30, 60, and 120 min relative to infusions and on d 6 the remaining 6 pigs/treatment were weighed, sacrificed, and their intestines were collected for later analyses. data were analyzed as a mixed-effect model with pig treated as random variable. compared to control, cdc at 60 mg/kg bw increased (p < 0.05) mean plasma glp-2 by 77%, small intestine length, intraepithelial lymphocytes and cleaved caspase in the ileum; tended to increase ileum weight and length (p < 0.08) and mean plasma glp-1 (p < 0.13) without affecting (p > 0.8) intake and final bw. at 120 mg/kg bw, cdc also increased (p < 0.05) glp-1 and glp-2, but reduced intake by about 50% and reduced bw as well as ileal crypt depth. other treatments did not affect measured parameters, except that bse tended to depress glp-1 (27%) and glp-2 (42%) compared to control. in conclusion, oral cdc treatment potently enhanced glp-2 secretion in weanling piglets, but the mitigation of weaning-induced intestinal atrophy was apparently counterbalanced by increased inflammation and reduced feed intake.