category: feed additives
authors: g. kühn, k. pallauf, j. garcía, j. segura, i. ipharraguerre, a. mereu, i. stubhaug, w. koppe, g. rimbach, d. menoyo
book/journal:aquaculture nutrition, 10.1111/anu.12440, 2016
this study aimed to test the use of chlorogenic acid (cga), a natural antioxidant, which might act improving the retention of eicosapentaenoic (epa) and docosahexaenoic (dha) fatty acids. fish were fed one of three experimental diets. a diet with 160 g kg−1 fish oil (fo) and 80 g kg−1vegetable oil (vo) was used as a positive control with high n-3 long-chain polyunsaturated fatty acids (lc)-pufa, a diet containing 40 g kg−1 fo and 200 g kg−1 vo was used as a negative control for low n-3 lc-pufa and 1 g kg−1 cga was added to the negative control diet to test the impact of cga on n-3 lc-pufa synthesis. the expression of genes involved in pufa synthesis was upregulated in the liver of fish fed the low n-3 lc-pufa diets. this might be due to the activation of sterol regulatory element-binding transcription factor 1 (srebp1) and liver x receptor. moreover, malondialdehyde concentration tended to decrease, and accumulation of dietary epa in the liver in relation to diet was observed in fish fed the low n-3 lc-pufa diets. however, the concentration of epa and dha was higher in fish fed the positive control diet. the inclusion of 1 g kg−1 cga did not exert any additional effect on lipid peroxidation, epa and dha concentrations or lc-pufa metabolism.