lucta – aromas, fragancias, zootecnia.-捕鱼游戏

enteral bile acids modulate intestinal immune response and gut microbiota in early-weaned piglets challenged with lps

categoría: feed additives

fecha:june 2015

autores: a. mereu, n. de diego-cabero, j.j. pastor, d.menoyo, i.r. ipharraguerre

libro/revista:j. anim. sci. vol. 93, suppl. s3/j. dairy sci. vol. 98, suppl. 2.

resumen:

bile acids (ba) have recently emerged as regulators of intestinal immune homeostasis and mucosal integrity. we examined the effects of administering enterally deoxycholic acid (dca) to early-weaned pigs challenged with lipopolysaccharide (lps) on gut ba profile, immune response and microbiota. twenty-four piglets were weaned at 21 d, acclimatized for 14 d, and subsequently grouped (n = 8) to be intragastrically infused with either deionized water (c , c−) or 15 mg of dca·kg−1 initial bw (dca) daily during 14 d. on d 28, c and dca piglets were injected i.p. with 150 µg lps·kg−1 bw. three h later, all animals were bled and killed for organ measurement and sampling. blood samples were analyzed for endotoxin (edt), interleukin (il)-6, and tumor necrosis factor α (tnf-α). expression of occludin, il-6 and il-10 genes and the concentration of individual ba were measured in the ileal mucosa. colonic microbiota was characterized by sequencing bacterial 16s ribosomal-rna. individual bw and feed intake were recorded weekly. data were analyzed with a mixed-effects model in which pig was treated as random effect and treatment as fixed effect. compared with c-, lps decreased total ba concentration (p < 0.01) and increased plasma tnf-α (p < 0.01) and edt (p < 0.02) as well as hepatic (p < 0.01) and intestinal (p < 0.03) weight. interestingly, dca infusion increased the proportion of ba with the greatest ability to induce ba-signaling pathways (p < 0.05), prevented alterations (p > 0.10) in plasma edt and intestinal weight, decreased expression of il genes (p < 0.04) in the ileum, and enhanced (p < 0.05) feed intake. in addition, treating pigs with dca increased (p < 0.05) peptostreptococcaceae and clostridiaceae whereas decreased (p < 0.05) lactobacillaceae in the colon. in conclusion, dca acted locally to prevent lps-induced inflammation and barrier disruption of the intestinal mucosa. these effects were associated with changes in the intestinal ba signature.